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KMID : 0903520040470020164
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2004 Volume.47 No. 2 p.164 ~ p.169
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Screening of Antagonistic Actinomycetes for Potato Scab Control and Isolation of Antibiotic Compound
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Lee Hyang-Burm
Cho Jong-Wun
Lim Chi-Hwan
Kim Chang-Jin
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Abstract
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In the course of our screening for biocontrol agent (BCA) against Streptomyces scabiei and S. turgidiscabies causing potato scab using 5,000 actinomtcete isolates, 9 antagonistic strains were selected as BCA candidates through in vitro and in vivo assay. An antagonistic strain, A020645 was highly resistant to some pesticides and antibiotics such as dazomet and mancozeb and showed high control value in vivo. Two bioactive compounds (compound A, B) were purified by anion exchange chromatography, solid phase (ODS) extraction, TLC and reverse phase HPLC. Their chemical structures are now thought to be nucleoside derivative as determined by 1H-NMR data analysis. Their full chemical structures would be elucidated through 13C-NMR, HMQC and HMBC analyses. Further studies will be focused on fitness in soil and formulation of the BCA candidates.
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KEYWORD
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potato, potato common scab, BCA, Streptomyces scabiei, Streptomyces turgidiscabies, bioactive compounds
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